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Technische Regel
ONR CEN/TS 15634-2
Issue date: 2012 03 15
Foodstuffs - Detection of food allergens by molecular biological methods - Part 2: Celery (Apium graveolens) - Qualitative determination of a specific DNA sequence in cooked sausages by real-time PCR (CEN/TS 15634-2:2012)
This Technical Specification specifies a method for the qualitative detection of celery (Apium graveolens) in
emulsion-type sausages (e.g. Frankfurter, Wiener).
Real-ti...
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Withdrawn
: 2019 11 15
Publisher:
Austrian Standards International
Format:
Digital | 20 Pages
Language:
German
| English
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Currently valid:
Optionally co-design standards:
This Technical Specification specifies a method for the qualitative detection of celery (Apium graveolens) in
emulsion-type sausages (e.g. Frankfurter, Wiener).
Real-time PCR detection of celery is based on an 101 bp (base pair) sequence from the gene of the mannitol
dehydrogenase (GenBank Acc. No. AF067082) of celery (Apium graveolens).
The method has been validated on emulsion-type sausages (Bavarian ¿Leberkäse¿) spiked with celery. For
this purpose meat batter containing mass fractions of 50 % pork meat, 25 % pork fat, 23 % crushed ice and
1,8 % of a mixture of sodium chloride, nitrite, nitrate, phosphates and ascorbates was prepared according to a
standard procedure for emulsion-type sausage. The meat batter was spiked with either ground celery seeds
or celery root powder to 1000 mg/kg. Lower spiking levels were obtained by diluting with celery-free meat
batter. The batter was stuffed into casings and heated at 65 °C for 60 min.
ÖNORM EN 15634-2
2019 11 15
Foodstuffs - Detection of food allergens by molecular biological methods - Part 2: Celery (Apium gra...
Norm
ONR CEN/TS 15634-2
2012 03 15
Foodstuffs - Detection of food allergens by molecular biological methods - Part 2: Celery (Apium gra...
Technische Regel
↖
ONR CEN/TS 15634-2
2011 08 15
Foodstuffs - Detection of food allergens by molecular biological methods - Part 2: Celery (Apium gra...
Technische Regel Entwurf
Norm
ÖNORM EN 15634-2
Issue date :
2019 11 15
Foodstuffs - Detection of food allergens by molecular biological methods - Part 2: Celery (Apium graveolens) - Detection of a specific DNA sequence in cooked sausages by real-time PCR