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Norm
ÖNORM EN ISO 17601
Issue date: 2018 08 15
Soil quality - Estimation of abundance of selected microbial gene sequences by quantitative PCR from DNA directly extracted from soil (ISO 17601:2016)
This International Standard specifies the crucial steps of a quantitative real-time polymerase chain reaction (qPCR) method to measure the abundance of selected microbial...
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Publisher:
Austrian Standards International
Format:
Digital | 42 Pages
Language:
German
| English
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This International Standard specifies the crucial steps of a quantitative real-time polymerase chain reaction (qPCR) method to measure the abundance of selected microbial gene sequences from soil DNA extract which provides an estimation of selected microbial groups.
It is noteworthy that the number of genes is not necessarily directly linked to the number of organisms that are measured. For example, the number of ribosomal operon is ranging from one copy to 20 copies in different bacterial phyla. Therefore, the number of 16S rRNA sequences quantified from soil DNA extracts does not give an exact estimate of the number of soil bacteria. Furthermore, the number of sequences is not necessarily linked to living microorganisms and can comprise sequences amplified from dead microorganisms.
ÖNORM EN ISO 17601
2018 08 15
Soil quality - Estimation of abundance of selected microbial gene sequences by quantitative PCR from...
Norm
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Norm
ISO 10381-6:2009
Issue date :
2009 03 03
Soil quality — Sampling — Part 6: Guidance on the collection, handling and storage of soil under aerobic conditions for the assessment of microbiological processes, biomass and diversity in the laboratory
Norm
ISO 17601:2016
Issue date :
2016 01 12
Soil quality — Estimation of abundance of selected microbial gene sequences by quantitative PCR from DNA directly extracted from soil