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Norm
ÖNORM EN 16936
Issue date: 2017 06 15
Animal feeding stuffs: Methods of sampling and analysis - Screening on the antibiotics tylosin, virginiamycin, spiramycin, bacitracin-zinc and avoparcin at sub-additive levels in compound feed by a microbiological plate test
This European Standard presents a method describing the screening on the antibiotics tylosin, virginiamycin, spiramycin, bacitracin-zinc and avoparcin at sub-additive lev...
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Publisher:
Austrian Standards International
Format:
Digital | 17 Pages
Language:
German
| English
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This European Standard presents a method describing the screening on the antibiotics tylosin, virginiamycin, spiramycin, bacitracin-zinc and avoparcin at sub-additive levels in complete feeding stuffs and milk replacers by a microbiological 3-plate test.
The limit of detection of the method is 1 mg/kg for avoparcin, tylosin, spiramycin and virginiamycin, and 5 mg/kg for zinc bacitracin. The presence of other (veterinary) antibiotics may interfere with the method.
Furthermore, high concentrations of metals (Cu, Zn) may interfere. The method should be used as a qualitative screening method. Positive results can be analysed further by TLC; for confirmatory purposes LC-MS is required.
A lower limit of detection for zinc bacitracin (3 mg/kg) is achievable, but should be established with an in house validation first.
ÖNORM EN 16936
2017 06 15
Animal feeding stuffs: Methods of sampling and analysis - Screening on the antibiotics tylosin, virg...
Norm
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