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ONR CEN/TS 17781

Issue date: 2022 10 01

Organic, organo-mineral and inorganic fertilizers - Detection of Escherichia coli

This document is applicable to fertilizing products, which are classified as PFC 1(A) and PFC 1(B) or the PFC 1(A) and PFC 1(B) component in PFC 7 of Regulation (EU) 2019/1009. However, the present method was not validated for blends. This document specifies a colony-count technique at 44 °C on a solid medium containing a chromogenic ingredient for the detection of the enzyme ß-glucuronidase. The method is based on ISO 16649-2. Strains of Escherichia coli which do not grow at 44 °C and, in particular, those that are ß-glucuronidase negative, such as Escherichia coli O157, will not be detected. Detected microorganisms are presumptively determined ß-glucuronidase-positive Escherichia coli, since some Enterobacteriaceae, in particular Shigella and Salmonella, can also show ß-glucuronidase activity at 44 °C.

General information on ONR CEN/TS 17781

Format
Digital | 22 Pages
Language
German | English | Download GER/ENG
Publisher
Austrian Standards International

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