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Technische Regel Entwurf

ONR CEN ISO/TS 15216-2

Issue date: 2012 12 15

Microbiology of food and animal feed - Horizontal method for detection of hepatitis A virus and norovirus in food using real-time RT-PCR - Part 2: Method for qualitative detection (ISO/DTS 15216-2:2012)

This part of ISO/TS 15216 describes a method for qualitative detection of HAV and NoV genogroups I (GI) and II (GII), from test samples of foodstuffs or food surfaces. F...
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Withdrawn : 2013 10 01
Publisher:
Austrian Standards International
Format:
Digital | 35 Pages
Language:
German | English | Download GER/ENG
This part of ISO/TS 15216 describes a method for qualitative detection of HAV and NoV genogroups I (GI) and II (GII), from test samples of foodstuffs or food surfaces. Following liberation of viruses from the test sample, viral RNA is then extracted by lysis with guanidine thiocyanate and adsorption on silica. Target sequences within the viral RNA are amplified and detected by real-time RT-PCR. This approach is also relevant for detection of the target viruses on fomites, or of other human viruses in foodstuffs, on food surfaces or on fomites following appropriate validation and using target-specific primer and probe sets.
ÖNORM EN ISO 15216-2
2019 12 15
Microbiology of the food chain - Horizontal method for determination of hepatitis A virus and norovi...
Norm
ONR CEN ISO/TS 15216-2
2013 10 01
Microbiology of food and animal feed - Horizontal method for determination of hepatitis A virus and ...
Technische Regel
ONR CEN ISO/TS 15216-2
2012 12 15
Microbiology of food and animal feed - Horizontal method for detection of hepatitis A virus and noro...
Technische Regel Entwurf
Technische Regel
ONR CEN ISO/TS 15216-2
Issue date : 2013 10 01
Microbiology of food and animal feed - Horizontal method for determination of hepatitis A virus and norovirus in food using real-time RT-PCR - Part 2: Method for qualitative detection (ISO/TS 15216-2:2013, Corrected Version 2013-05-01)