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Technische Regel Entwurf
ONR CEN ISO/TS 13136
Issue date: 2012 04 15
Microbiology of food and animal feed - Real-time polymerase chain reaction (PCR)-based method for the detection of food-borne pathogens - Horizontal method for the detection of Shiga toxin-producing Escherichia coli (STEC) belonging to O157, O111, O26, O103 and O145 serogroups (ISO/DTS 13136:2012)
This Technical Specification describes a horizontal method for the detection of:
a) the major virulence genes of STEC;
b) the genes associated with the serogroups O157,...
Withdrawn: 2013 04 01
Publisher:
Austrian Standards International
Format:
Digital | 33 Pages
Language:
German
| English
| Download GER/ENG
Currently valid:
Optionally co-design standards:
ICS
This Technical Specification describes a horizontal method for the detection of:
a) the major virulence genes of STEC;
b) the genes associated with the serogroups O157, O111, O26, O103, and O145.
In the case of the detection of these genes, the isolation of the strain is attempted to confirm the simultaneous
presence of the genes in the same live bacterial cell.
This Technical Specification is based on the use of real-time PCR as reference technology for the detection
of the virulence and serogroup-associated genes. Therefore a real-time PCR protocol is described in detail.
This Technical Specification is applicable to:
1) products intended for human consumption and the feeding of animals;
2) environmental samples in the area of food production and food handling;
3) environmental samples in the area of primary production.
ONR CEN ISO/TS 13136
2013 04 01
Microbiology of food and animal feed - Real-time polymerase chain reaction (PCR)-based method for th...
Technische Regel
ONR CEN ISO/TS 13136
2012 04 15
Microbiology of food and animal feed - Real-time polymerase chain reaction (PCR)-based method for th...
Technische Regel Entwurf
↖
Technische Regel
Issue date :
2013 04 01
Microbiology of food and animal feed - Real-time polymerase chain reaction (PCR)-based method for the detection of food-borne pathogens - Horizontal method for the detection of Shiga toxin-producing Escherichia coli (STEC) and the determination of O157, O111, O26, O103 and O145 serogroups (ISO/TS 13136:2012)