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ÖNORM EN ISO 9308-4

Issue date: 2025 12 15

Water quality - Enumeration of Escherichia coli and coliform bacteria - Part 4: Membrane filtration method for Escherichia coli in water with high levels of background bacteria (ISO/DIS 9308-4:2025)

This document describes a highly specific method for the enumeration of Escherichia coli (E. coli) in water. The method is based on membrane filtration, subsequent culture at 44 °C on a chromogenic agar medium containing a chromogenic ingredient for the detection of the enzyme ß-glucuronidase, and calculation of the number of target organisms in the sample. Because of the high specificity of the method, this document is suitable for waters with high levels of background bacteria, such as surface waters including bathing water and wastewater. E. coli strains which do not grow at 44 °C and those that are β-glucuronidase negative, such as E. coli O157, will not be detected as E. coli by this method.

General information on ÖNORM EN ISO 9308-4

Format
Digital | 22 Pages
Language
German | English | Download GER/ENG
Publisher
Austrian Standards International

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ÖNORM EN ISO 9308-4 - Water quality - Enumeration of Escherichia coli and coliform bacteria - Part 4: Membrane filtration method for Escherichia coli in water with high levels of background bacteria (ISO/DIS 9308-4:2025)

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