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STANDARD

Current

ÖNORM EN ISO 17174

Issue date: 2024 12 15

Molecular biomarker analysis - DNA barcoding of fish and fish products using defined mitochondrial cytochrome b and cytochrome c oxidase I gene segments (ISO 17174:2024)

This document specifies a method for the identification of single fish and fish fillets to the level of genus or species. It allows the identification of a large number of commercially important fish species using DNA barcoding. This method was validated on raw fish. Laboratory experience indicates additional applicability to processed fish products (e.g. cold smoked, hot smoked, salted, frozen, cooked, fried and deep-fried samples). The described method is usually unsuitable for the analysis of highly processed foods (e.g. tins of fish with highly degraded DNA where the fragment lengths are not sufficient for amplification of the targets). Furthermore, it does not apply to complex fish products containing mixtures of two or more fish species. The identification of fish species is carried out by PCR amplification of either a segment of the mitochondrial cytochrome b gene (cytb) or the cytochrome c oxidase I gene (cox1, syn COI), or both, followed by sequencing of the PCR products and subsequent sequence comparison with entries in databases.

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General information on ÖNORM EN ISO 17174

Format

Paper | 29 Pages

Language

German | English | Download GER/ENG

Publisher

Austrian Standards International

Committee 205 Food and animal feeding stuffs analysis

Timeline

An overview of the development of ÖNORM EN ISO 17174 from 2019 til today.

• ÖNORM EN ISO 17174

  2024 12 15 Standard ÖNORM EN ISO 17174, Edition: 2024 12 15

  Molecular biomarker analysis - DNA barcoding of fish and fish products using defined mitochondrial c...
• ONR CEN/TS 17303

  2019 08 15 Technische Regel ONR CEN/TS 17303, Edition: 2019 08 15

  Foodstuffs - DNA barcoding of fish and fish products using defined mitochondrial cytochrome b and cy...

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Normative References

Here you will find an extract of the normative references. Please refer to the document itself for the full list.

Dokumentnummer	Titel	Ausgabedatum	Art	Link
Dokumentnummer ISO 16577:2016	Titel Molecular biomarker analysis — Terms and definitions	Ausgabedatum 2016 03 16	Art Standard	Zur Produktseite
Dokumentnummer ISO 20813:2019	Titel Molecular biomarker analysis — Methods of analysis for the detection and identification of animal species in foods and food products (nucleic acid-based methods) — General requirements and definitions	Ausgabedatum 2019 05 09	Art Standard	Zur Produktseite

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