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STANDARD

Current

ÖNORM EN ISO 15216-1

Issue date: 2021 10 01

Microbiology of the food chain - Horizontal method for determination of hepatitis A virus and norovirus using real-time RT-PCR - Part 1: Method for quantification (ISO 15216-1:2017 + Amd 1:2021) (consolidated version)

This document specifies a method for the quantification of levels of HAV and norovirus genogroup I (GI) and II (GII) RNA, from test samples of foodstuffs (soft fruit, leaf, stem and bulb vegetables, bottled water, BMS) or food surfaces. Following liberation of viruses from the test sample, viral RNA is then extracted by lysis with guanidine thiocyanate and adsorption on silica. Target sequences within the viral RNA are amplified and detected by real-time RT-PCR. This method is not validated for detection of the target viruses in other foodstuffs (including multi-component foodstuffs), or any other matrices, nor for the detection of other viruses in foodstuffs, food surfaces or other matrices.

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General information on ÖNORM EN ISO 15216-1

Format

Digital | 60 Pages

Language

German | English | Download GER/ENG

Publisher

Austrian Standards International

Committee 205 Food and animal feeding stuffs analysis

Timeline

An overview of the development of ÖNORM EN ISO 15216-1 from 2013 til today.

• ÖNORM EN ISO 15216-1

  2021 10 01 Standard ÖNORM EN ISO 15216-1, Edition: 2021 10 01

  Microbiology of the food chain - Horizontal method for determination of hepatitis A virus and norovi...
• ÖNORM EN ISO 15216-1

  2017 07 01 Standard ÖNORM EN ISO 15216-1, Edition: 2017 07 01

  Microbiology of the food chain - Horizontal method for determination of hepatitis A virus and norovi...
• ONR CEN ISO/TS 15216-1

  2013 10 01 Technische Regel ONR CEN ISO/TS 15216-1, Edition: 2013 10 01

  Microbiology of food and animal feed - Horizontal method for determination of hepatitis A virus and ...

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Normative References

Here you will find an extract of the normative references. Please refer to the document itself for the full list.

Dokumentnummer	Titel	Ausgabedatum	Art	Link
Dokumentnummer ISO 20838:2006	Titel Microbiology of food and animal feeding stuffs — Polymerase chain reaction (PCR) for the detection of food-borne pathogens — Requirements for amplification and detection for qualitative methods	Ausgabedatum 2006 04 06	Art Standard	Zur Produktseite
Dokumentnummer ISO 22119:2011	Titel Microbiology of food and animal feeding stuffs — Real-time polymerase chain reaction (PCR) for the detection of food-borne pathogens — General requirements and definitions	Ausgabedatum 2011 07 08	Art Standard	Zur Produktseite
Dokumentnummer ISO 22174:2005	Titel Microbiology of food and animal feeding stuffs — Polymerase chain reaction (PCR) for the detection of food-borne pathogens — General requirements and definitions	Ausgabedatum 2005 03 02	Art Standard	Zur Produktseite
Dokumentnummer ISO 7218:2007	Titel Microbiology of food and animal feeding stuffs — General requirements and guidance for microbiological examinations	Ausgabedatum 2007 08 02	Art Standard	Zur Produktseite

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