STANDARD

Current

ÖNORM EN ISO 11063

Issue date: 2021 04 15

Soil quality - Direct extraction of soil DNA (ISO 11063:2020)

The present document specifies a method for direct extraction of DNA from soil samples to analyse the abundance and composition of microbial communities by various techniques of molecular biology including real-time quantitative PCR (qPCR). This method is mainly dedicated to agricultural and forest soils. This method can possibly not be suitable for soils rich in organic matter (e.g. peat soils) or soils heavily polluted with organic pollutants or heavy metals. The direct extraction of DNA from soil samples provides unique insight into the alpha- and beta-diversity of microbial communities. Next-generation sequencing of amplicons obtained by PCR (polymerase chain reaction) amplification of soil DNA constitutes a promising domain which will in the near future contribute to the development of routine tools to monitor microbial communities in soil environments.

General information on ÖNORM EN ISO 11063

Format
Paper | 16 Pages
Language
German | English | Download GER/ENG
Publisher
Austrian Standards International

Timeline

An overview of the development of ÖNORM EN ISO 11063 from 2013 til today.

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Normative References

Here you will find an extract of the normative references. Please refer to the document itself for the full list.

Dokumentnummer Titel Ausgabedatum Art Link
Dokumentnummer
ISO 18400-206:2018
Titel
Soil quality — Sampling — Part 206: Collection, handling and storage of soil under aerobic conditions for the assessment of microbiological processes, biomass and diversity in the laboratory
Ausgabedatum
2018 10 19
Art
Standard

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ÖNORM EN ISO 11063 - Soil quality - Direct extraction of soil DNA (ISO 11063:2020)

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