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ÖNORM EN 17547

Issue date: 2022 03 15

Animal feeding stuffs - Methods of sampling and analysis - Determination of vitamin A, E and D content - Method using solid phase extraction (SPE) clean-up and high-performance liquid chromatography (HPLC)

This document specifies a method for the determination of the content of the total vitamin A (retinol), vitamin E (a-tocopherol) and vitamin D3 (cholecalciferol) in animal feed using solid phase extraction (SPE) clean-up and high-performance liquid chromatography (HPLC). The method has been successfully tested in collaborative trial for complete feed for broilers, pigs, and turkey, for premixture for broilers and piglets, for complementary feed for cows and mineral feed within the following ranges: - vitamin A: 4 365 IU/kg – 4 118 352 IU/kg; - vitamin E: 22 mg/kg – 13 800 mg/kg; - vitamin D3: 1 668 IU/kg – 1 638 150 IU/kg. The limits of quantification were not determined within the validation study. Quantification limits of 1 100 IU for vitamin A/kg (using UV-detection), 4 mg for vitamin E/kg (using UV-detection), 2 mg for vitamin E/kg (using fluorescence detection) and 2 000 IU for vitamin D/kg (using UV-detection) should be normally achieved. Lower limits are possible provided they are validated by the user.

General information on ÖNORM EN 17547

Format
Digital | 43 Pages
Language
German | English | Download GER/ENG
Publisher
Austrian Standards International

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ÖNORM EN 17547 - Animal feeding stuffs - Methods of sampling and analysis - Determination of vitamin A, E and D content - Method using solid phase extraction (SPE) clean-up and high-performance liquid chromatography (HPLC)

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